Objective: To determine and compare vitamin E content in five different regions of Amygdalus mongolica. Method: HPLC method was established for determining the content of vitamin E. Methanol-water (98:2) was used as the mobile phase at the flow rate of 1.00 mL·min-1. The chromatographic column was WondaSil-C18 (4.6 mm×250 mm

5 μm); the detection wavelength was set at 300 nm; the column temperature was kept at 25℃

and the ethanol was used as solvents. Result: The calibration curves of vitamin E was in good linearity over the range of 1-100 μg (r=0.999 6). The average recovery of vitamin E was 100.1% (n=3

RSD 1.740). Among five samples from different areas

the highest content of vitamin E is determined in the sample of Yabuge Gebi and the lowest is the one from Guyang Payushu. Conclusion: The method was proved to be rapid

accurate and credible.