LI Shu-qing, XIAO Xiao, WANG Kun-feng, et al. Method for Determination of Total Saponins in from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 118-120.
LI Shu-qing, XIAO Xiao, WANG Kun-feng, et al. Method for Determination of Total Saponins in from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 118-120. DOI: 10.11653/syfj2013120118.
Objective: The method for determination of total saponins in Dioscoreae hypoglauca from different areas was developed. Method: Spectrophotometer was used to determine the total saponins with perchloric acid for colorimetric reaction and diosgenin as the reference substance at 408 nm as measurement wavelength. Result: The linearity range of diosgenin was 1.55-12.42 mg·L-1(r=0.999 5). The average recovery was 100.69%
RSD 2.75%. The content of total saponins in D. hypoglauca from different areas was 2.85% (Yulin
Guangxi)
2.76% (Nanning
Guangxi)
2.54% (Linan
Zhejiang)
1.94% (Yinchang
Hubei)
0.84% (Chongzhou
Sichuan) respectively. Conclusion: The method was simple
accurate and reproducible
which could be applied to the determination of saponins in D. hypoglauca from different areas.