Objective: To study the change of total ginkgolic acids in Ginkgo biloba leaves of different habitats. Method: The content of total ginkgolic acids in G. biloba leaves was determined by HPLC. A Hypersil C18 column (4.6 mm×250 mm

5 μm) and the mobile phase of methanol and 1% acetic acid (90:10) were used; the flow rate was 1.0 mL·min-1

the wavelength was set at 310 nm. The content was calculated with external standard method. Result: The content of total ginkgoic acids in G. biloba leaves varied with different habitats. The content in the samples from Tancheng was the highest

while Pizhou is the lowest. Conclusion: The content of total ginkgolic acids in G. biloba leaves from different habitats is markedly different. We should have a choice in use.