愈肾颗粒不同药对配伍对肾小球系膜细胞基质金属蛋白酶-2及其抑制剂-2的影响

杨冠琦; 丁晓欢; 岳志军; 张少卿; 张君

doi:10.11653/syfj2013120169

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愈肾颗粒不同药对配伍对肾小球系膜细胞基质金属蛋白酶-2及其抑制剂-2的影响

更新时间：2024-01-04

- 愈肾颗粒不同药对配伍对肾小球系膜细胞基质金属蛋白酶-2及其抑制剂-2的影响
- Effect of Different Couplet Medicines from Yushen Granules on Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Mesangial Cell
- 中国实验方剂学杂志 2013年19卷第12期页码：169-171
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  国家科技重大专项项目(2009ZX09103)
- DOI：10.11653/syfj2013120169
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- 纸质出版日期：2013
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杨冠琦, 丁晓欢, 岳志军, 等. 愈肾颗粒不同药对配伍对肾小球系膜细胞基质金属蛋白酶-2及其抑制剂-2的影响[J]. 中国实验方剂学杂志, 2013,19(12):169-171.

YANG Guan-qi, DING Xiao-huan, YUE Zhi-jun, et al. Effect of Different Couplet Medicines from Yushen Granules on Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Mesangial Cell[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 169-171.
杨冠琦, 丁晓欢, 岳志军, 等. 愈肾颗粒不同药对配伍对肾小球系膜细胞基质金属蛋白酶-2及其抑制剂-2的影响[J]. 中国实验方剂学杂志, 2013,19(12):169-171. DOI： 10.11653/syfj2013120169.

YANG Guan-qi, DING Xiao-huan, YUE Zhi-jun, et al. Effect of Different Couplet Medicines from Yushen Granules on Matrix Metalloproteinase-2 and Tissue Inhibitor of Metalloproteinase-2 in Mesangial Cell[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 169-171. DOI： 10.11653/syfj2013120169.

摘要

目的: 考察黄芪、太子参(益气摄血组)

牡丹皮、丹参(凉血化瘀组)

紫草、益母草(解毒化瘀组)3组药对对大鼠肾小球系膜细胞基质金属蛋白酶-2(MMP-2)及基质金属蛋白酶抑制剂-2(TIMP-2)m-RNA表达的影响。方法: 应用血清药理学方法

制取中药药对的含药血清。以血管紧张素Ⅱ(Ang Ⅱ)作为刺激因子

培养大鼠肾小球系膜细胞

使其发生增殖。应用反转录多聚酶链反应技术(RT-PCR)观察各组间MMP-2

TIMP-2的基因表达情况。结果: 益气摄血组对TIMP-2抑制作用显著

与模型组比较有显著性差异;其他2组虽有抑制趋势

但无统计学意义。3组药对对MMP-2的影响均无统计学意义。结论: 黄芪、太子参可通过抑制TIMP-2的基因表达

发挥防治肾小球硬化的作用。

Abstract

Objective: To investigate effect of Astragali Radix and Pseudostellariae Radix

Cortex Moutan Radicis and Salviae Miltiorrhizae Radix et Rhizoma

Arnebiae Radix and Leonuri Herba on m-RNA expression of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) in mesangial cell of rats. Method: Using serum phamacological method

we got harmaeologieal serum of 3 couplet medicines.Cultured the glomerular mesangial cells and prolifelated them with angiotensin Ⅱ as stimulating factor.m-RNA expression of MMP-2 and TIMP-2 were measured by RT-PCR. Result: Effect of these 3 couplet medicines on MMP-2 were not statistically significant.Group of Astragali Radix and Pseudostellariae Radix obviously inhibited expression of TIMP-2

and there was significant difference by comparing with the modle group;Other groups also could inhibite the expression of TIMP-2

but there were not statistically significant. Conclusion: Couplet medicines of Astragali Radix and Pseudostellariae Radix could play prevention and treatment roles of glomeruselerosis by inhibiting gene expression of TIMP-2.

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