Objective: To investigate the elevating expression of Bcl-2 by anemarrhena total saponins on beta-amyloid (Aβ)-induced PC12 cell injury. Method: PC12 cells were randomly divided into 6 group as following:normal group

model group

anemarrhena total saponins groups(5

10

20 mg·L-1)

the donepezil HCl group. Excluding the normal group

the PC12 cells in others groups were exposed to DMEM containing beta-amyloid (20 μmol·L-1) and anemarrhena total saponins groups(5

10

20 mg·L-1

the donepezil HCl(1 μmol·L-1).The mRNA and protein expression level of the Bcl-2 were detected using semi-quantitative reverse transcriptase PCR (RT-PCR) and western blot respectively. The cell survival were measured by MTT methods. Result: Anemarrhena total saponins significantly attenuated Aβ-induced PC12 cell injury

and elevated the mRNA and protein expression of Bcl-2

compared with model group(P<0.05). Conclusion: Anemarrhena total saponins can inhibit PC12 cell injury induced by Aβ25-35

and the mechanism maybe involving in increasing

the expression levels of Bcl-2 mRNA and protein.