Objective: To explore the protective effects of Guanxin Shutong capsules (GSC) and its single herbs on hypoxia/reoxygenation (H/R) injury and proliferaion in primary cultured myocardial cells and aortal smooth muscle cells. Method: The protective effects of six groups of drugs with four carnitine concentrations (0.01

0.05

0.10

0.20 μg·L-1)

including Choerospondia axillaris extracts of Salvia miltiorrhizae

water extracts of Bambusa texeilis

alcohol-extract B. texeilis

Borneol and GSC

on the proliferation of myocardial cells and aortal smooth muscle cells induced by Angiotension Ⅱ were detected with MTT colorimetric method. (H/R) cell model was established by cultured primary myocardial cells and aortal smooth muscle cells of neonatal rats. The cells were divided into normal control group

H/R group and administration group(0.01

0.05

0.10 μg·L-1)

after 24 hours

lactate dehydrogenase (LDH)

malondialchehyche (MDA) and superoxide dismutase (SOD)

nitric oxide (NO) and nitric oxide synthase (NOS) were measured. Result: Compared with the normal control group

the survival rate of cells

SOD

NO and NOS in H/R group were obviously decreased

while LDH

MDA were obviously increased. Compared with the H/R group

the survival rate of cells

SOD

NO and NOS in administration group were obviously increased

while LDH

MDA were obviously decreased. Conclusion: GSC and its single herbs can protect myocardial cells and aortal smooth muscle cells injury induced by H/R

and the mechanism may be related with the effects on anti-lipid peroxidation and vasodilation of blood vessels and inhibition of cell proliferation.