Objective: To observe the impacts on protein and mRNA expression of MMP-1

MMP-2

MMP-9

collagen I

collagenⅢ and explore the mechanisms of cardiac collagen fibers. Method: Sixty 24\|week\|old SHR were randomly divided into the model group

perindopril group(0.4 mg·kg-1)

perindopril associated with Qishen Jianpi decoction group(0.4 mg·kg-1+20 g·kg-1)

and another 3 groups individually high

middle low dose of Qishen Jianpi decoction(40

20

10 g·kg-1)

the normal group is 10 normal arterial pressure WKY at the same age and kind.Gastric infusion was administered continuously for 6 weeks

once per day and the forage feeding was applied normally.At the end of the 6th week

after anesthetized

the heart tissues were separated.Immunohistochemical (IHC) method was to the protein expression of collagen I and collagen Ⅲ;RT-PCR was to the content of mRNA and Western blot was to the protein expression of myocardial MMP-1

MMP-2

MMP-9 in each group. Result: The protein expression of collagen I and collagenⅢ was significantly decreased in perindopril group

perindopril associated with Qishen Jianpi decoction group and high dose group compared with model group (P<0.01

P<0.05)

and the expression of collagenⅢ was significantly decreased in middle dose group(P<0.05).The mRNA and the protein expression of MMP-1

MMP-2

MMP-9 was significantly increased in perindopril group

perindopril associated with Qishen Jianpi decoction group and high dose of Qishen Jianpi decoction compared with model group (P<0.01

P<0.05)

and the expression of MMP-1 was significantly increased in middle dose group(P<0.05). Conclusion: By regulating the level of MMPs and collagen

Qishen Jianpi decoction could improve the function of hearts

it may be one of the mechanisms prevent the development of arterial blood pressure in SHR and inhibit accumulation of cardiac collagen fibers.