三黄汤制炉甘石中盐酸小檗碱的含量测定

刘善新; 靳光乾; 王亮

doi:10.11653/syfj20131300107

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三黄汤制炉甘石中盐酸小檗碱的含量测定

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- 三黄汤制炉甘石中盐酸小檗碱的含量测定
- Content Determination of Berberine Hydrochloride in Prossing Calamina by Sanhuang Tang
- 中国实验方剂学杂志 2013年19卷第13期页码：107-109
- 作者机构：
- 作者简介：
- 基金信息：
  
  2012年版《山东省中药炮制规范》研究项目
- DOI：10.11653/syfj20131300107
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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刘善新, 靳光乾, 王亮. 三黄汤制炉甘石中盐酸小檗碱的含量测定[J]. 中国实验方剂学杂志, 2013,19(13):107-109.

LIU Shan-xin, JIN Guang-qian, WANG Liang. Content Determination of Berberine Hydrochloride in Prossing Calamina by Sanhuang Tang[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 107-109.
刘善新, 靳光乾, 王亮. 三黄汤制炉甘石中盐酸小檗碱的含量测定[J]. 中国实验方剂学杂志, 2013,19(13):107-109. DOI： 10.11653/syfj20131300107.

LIU Shan-xin, JIN Guang-qian, WANG Liang. Content Determination of Berberine Hydrochloride in Prossing Calamina by Sanhuang Tang[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 107-109. DOI： 10.11653/syfj20131300107.

摘要

目的: 建立三黄汤制炉甘石中盐酸小檗碱的含量测定方法。方法: 采用高效液相色谱法。lichrospher C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈-0.1%磷酸溶液(46∶64)(每100 mL含0.1 g十二烷基硫酸钠)

流速1 mL·min-1

柱温30 ℃

检测波长350 nm。结果: 盐酸小檗碱的进样量在0.042 0~0.525 μg线性关系良好

r=0.999 8

平均回收率为95.24%

RSD 1.33%。3批三黄汤制炉甘石中盐酸小檗碱的含量分别为0.326%

0.347%

0.373%。结论: 方法准确、灵敏、重复性好

可作为三黄汤制炉甘石中盐酸小檗碱的含量测定方法。

Abstract

Objective:To establish a method for the content determination of of berberine hydrochloride in prossing Calamina by Sanhuang Tang. Method: HPLC was used with lichrospher C18column (4.6 mm×150 mm

5μm)

acetonitrile-0.1% phosphoric acid (46:64) with mobile phase. The flow rate was 1 mL·min-1;the column temperature was maintained at 30 ℃;the detection wavelength was set at 350 nm. Result: Berberine hydrochloride had a good linearity within 0.042 0-0.525 μg (r=0.999 8) and the average recovery was 95.24% with RSD 1.33%. The content of berberine hydrochloride in three samples of prossing Calamina by Sanhuang Tang was 0.326%

0.347%

0.373%. Conclusion: This method is simple

accurate with good reproducibility

and can be used for the content determination of berberine hydrochloride in prossing Calamina by Sanhuang Tang.

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