Objective:To optimize particle size of ultrafine powder by investigating in vitro dissolution and receding jaundice effect of micronized Canhuang tablets(MCHP). Method: With berberine as index

release of berberine from MCHP with different crush size in different time periods was determined by HPLC

calculated cumulative dissolution

and then observed dissolution.At the same time

rats jaundice model was caused by 4%ANTI

after administered in accordance with dosing regimen

the femoral artery blood was collected for determining serum biochemical markers

such as ALT

AST

TBIL

DBIL and γ-GT

receding jaundice effect of MCHP with different grinding particle size was evaluated.Superfine powder particle size of MCHP was optimized by Comprehensive evaluating in vitro dissolution and receding jaundice effect. Result: With increasing of pulverized mesh

cumulative dissolution of berberine increased

cumulative dissolution of 200

300 mesh was 94.86%

96.18% at 60 min

respectively.For receding jaundice effect

Canhuang tablets with 200 mesh could significantly reduce ALT

AST and γ-GT

which was stronger than those of Canhuang tablets with 100

150 and 300 mesh

furthermore

that of 200 mesh was superior to control drug ursodeoxycholic acid tablets in reducing ALT and γ-GT.Comprehensive appearance of tablets and actual production

200 mesh was determined as optimum crush size. Conclusion: It was a feasible and convenient method to optimize ultrafine powder particle size of MCHP by combining in vitro dissolution and receding jaundice effect.