岗松总黄酮的纯化工艺优选

邱宏聪; 刘布鸣; 孙翠

doi:10.11653/syfj2013130034

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岗松总黄酮的纯化工艺优选

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- 岗松总黄酮的纯化工艺优选
- Optimization of Purification Process for Total Flavonoids from L.
- 中国实验方剂学杂志 2013年19卷第13期页码：34-36
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西壮族自治区卫生厅中医药科技专项(GZPT1229);广西中药质量标准研究重点实验室主任基金(桂中重科201105)
- DOI：10.11653/syfj2013130034
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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邱宏聪, 刘布鸣, 孙翠. 岗松总黄酮的纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(13):34-36.

QIU Hong-cong, LIU Bu-ming, SUN Cui. Optimization of Purification Process for Total Flavonoids from L.[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 34-36.
邱宏聪, 刘布鸣, 孙翠. 岗松总黄酮的纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(13):34-36. DOI： 10.11653/syfj2013130034.

QIU Hong-cong, LIU Bu-ming, SUN Cui. Optimization of Purification Process for Total Flavonoids from L.[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 34-36. DOI： 10.11653/syfj2013130034.

摘要

目的: 优选岗松总黄酮的大孔吸附树脂纯化工艺。方法: 以总黄酮吸附率和洗脱率为指标

采用UV测定总黄酮含量

筛选最佳大孔吸附树脂

并通过单因素试验考察岗松总黄酮的大孔树脂纯化工艺。结果: 采用AB-8型大孔吸附树脂对岗松总黄酮进行纯化

其最佳工艺条件为上样液生药质量浓度0.3 g·mL-1

上样流速1 BV·h-1

上样体积3 BV

加50%乙醇5 BV以2 BV·h-1的流速洗脱

总黄酮纯度达52.33%。结论: 优选的工艺合理可行

适用于工业化生产。

Abstract

Objective:To optimize purification process of total flavonoids from Baeckea frutescens L. by macroporous adsorption resin. Method: Different types of resin were selected with absorption and desorption ratio of total flavonoids as indexes

the content of total flavonoids was determined by UV

single factor test was adopted to investigate purification technology of total flavonoids by macroporous adsorption resin. Result: AB-8 type resin was adopted

its optimum process conditions were as followings:the concentration of sample solution 0.3 g·mL-1

absorption velocity 1 BV·h-1

sample volume 3 BV

eluted by 5 BV 50% ethanol at flow rate of 2 BV·h-1.Under these conditions

purity of total flavonoids was up to 52.33%. Conclusion: Optimized process was rational

feasible and suitable for industrial production.

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