UPLC-ESI-MS-MS分析生晒参和紫红参中皂苷类成分

邱楠楠; 刘金平; 苏航; 董刚; 刘海燕; 李平亚

doi:10.11653/syfj2013130091

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UPLC-ESI-MS-MS分析生晒参和紫红参中皂苷类成分

更新时间：2024-01-04

- UPLC-ESI-MS-MS分析生晒参和紫红参中皂苷类成分
- Analysis on Ginsenosides of Dry Radix Ginseng and Purplish Red Ginseng with UPLC-MS-MS
- 中国实验方剂学杂志 2013年19卷第13期页码：91-93
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省科技厅重大项目(20096039)
- DOI：10.11653/syfj2013130091
  中图分类号：
- 纸质出版日期：2013
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邱楠楠, 刘金平, 苏航, 等. UPLC-ESI-MS-MS分析生晒参和紫红参中皂苷类成分[J]. 中国实验方剂学杂志, 2013,19(13):91-93.

QIU Nan-nan, LIU Jin-ping, SU Hang, et al. Analysis on Ginsenosides of Dry Radix Ginseng and Purplish Red Ginseng with UPLC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 91-93.
邱楠楠, 刘金平, 苏航, 等. UPLC-ESI-MS-MS分析生晒参和紫红参中皂苷类成分[J]. 中国实验方剂学杂志, 2013,19(13):91-93. DOI： 10.11653/syfj2013130091.

QIU Nan-nan, LIU Jin-ping, SU Hang, et al. Analysis on Ginsenosides of Dry Radix Ginseng and Purplish Red Ginseng with UPLC-MS-MS[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 91-93. DOI： 10.11653/syfj2013130091.

摘要

目的: 采用UPLC-ESI-MS-MS方法比较生晒参和紫红参中人参皂苷类成分的种类及含量

阐明紫红参特异成分。方法: Waters ACQUITY UPLC BEH C18 (2.1 mm× 50 mm

1.7 μm) 色谱柱

流动相A (0.1%甲酸)-B (乙腈)梯度洗脱

柱温30 ℃;质谱条件:电喷雾离子化源(ESI)

负离子检测模式。结果: 与生晒参相比

紫红参中人参皂苷的种类和数量发生了改变

检测出含量较高的人参皂苷为20(S)-人参皂苷Rg3、20(R)-人参皂苷Rg3、20(R)-人参皂苷Rh1、20(R)-人参皂苷Rg2

Rk1

Rg5

Ro、姜状三七苷R1等。结论: 人参加工成紫红参后皂苷类成分发生较大的变化

产生了大量稀有皂苷

人参加工可能产生紫红参的特异成分。

Abstract

Objective:To clarify special constituents of purplish red ginseng

the kinds and content of ginsenosides in dry radix ginseng and purplish red ginseng were determined by UPLC-ESI-MS-MS method in this paper. Method: Waters ACQUITY UPLC BEH C18 (2.1 mm× 50 mm

1.7 μm) column was used with mobile phase consisted of 0.1% methanoic acid - acetonitrile (gradient elution). The column temperature was set at 30 ℃. MS conditions:ESI

the negative electrospray ionization mode. Result: Compared with the dry radix ginseng

the kinds and content of ginsenosides in purplish red ginseng had changed

the content of 20(S)-ginsenoside Rg3

20(R)-ginsenoside Rg3

20(R)-ginsenoside Rh1

20(R)-ginsenoside Rg2

Rk1

Rg5

Ro and zingibroside R1 increased in purplish red ginseng. Conclusion: Great changes of saponins have been obviously found in processing ginseng to purplish red ginseng. Special constituents of purplish red ginseng could be produced in ginseng processing.

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