HPLC同时测定清肺抑火丸中3个苷类成分

陈峻; 徐华; 陈小轩

doi:10.11653/syfj2013130134

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HPLC同时测定清肺抑火丸中3个苷类成分

更新时间：2024-01-04

- HPLC同时测定清肺抑火丸中3个苷类成分
- Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC
- 中国实验方剂学杂志 2013年19卷第13期页码：134-136
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- DOI：10.11653/syfj2013130134
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- 纸质出版日期：2013
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陈峻, 徐华, 陈小轩. HPLC同时测定清肺抑火丸中3个苷类成分[J]. 中国实验方剂学杂志, 2013,19(13):134-136.

CHEN Jun, XU Hua, CHEN Xiao-xuan. Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 134-136.
陈峻, 徐华, 陈小轩. HPLC同时测定清肺抑火丸中3个苷类成分[J]. 中国实验方剂学杂志, 2013,19(13):134-136. DOI： 10.11653/syfj2013130134.

CHEN Jun, XU Hua, CHEN Xiao-xuan. Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 134-136. DOI： 10.11653/syfj2013130134.

摘要

目的: 建立清肺抑火丸中3个苷类成分的含量标准。方法: 采用HPLC

同时以2个检测波长对清肺抑火丸中的黄芩苷、京尼平苷、京尼平龙胆二糖苷进行定量分析。Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm

5 μm);流动相甲醇(A)-1%甲酸溶液(B)-乙腈(C)梯度洗脱

检测波长238

278 nm。结果: 黄芩苷、京尼平苷、京尼平龙胆二糖苷分别在0.165~1.650

0.106~1.060

0.035~0.350 μg(r>0.999 7)线性关系良好

平均回收率>96.75%

RSD<2.05%。结论: 该方法同时进行黄芩苷、京尼平苷、京尼平龙胆二糖苷的定量分析

可作为清肺抑火丸的质量控制标准。

Abstract

Objective:To establish a HPLC method for determination of three constituents in Qingfei Yihuo Pills. Method: Baicalin

geniposide and genipin-1-O-β-gentiobioside were determined simultaneously by HPLC on a agilent Zorbax SB-C18 column with the methanol(A)-1.0% formic acid anhydrous(B)-acetonitrile(C) in gradient elution as the mobile phrase. The detection wavelength was set at 238

440 nm and the flow rate was 1.0 mL·min-1. Result: The standard curves for baicalin

geniposide and genipin-1-O-β-gentiobioside were linear within the range of 0.165-1.650

0.106-1.060

0.035-0.350 μg (r>0.999 7)

and the average recoveries were over 96.75% (RSD<2.05%). Conclusion: The method is simple and accurate and could be used for quality control of Qingfei Yihuo Pills.

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