Objective:To study the microstructure of stems and leaves of Artabotrys hexapetalus and distribution characteristics of endophytic fungi in the tissues

and to isolate and identify these endophytic fungi from the stem and the leaf of A. hexapetalus. Method: Permanent paraffin-cut section

optical microscope photography and histochemistry were used to study the location of endophytic fungi. The solidified potato dextrose agar(PDA) of plates was applied for the isolation of the endophytic fungi

and the identification was completed by the internal transcribed spacer (ITS) sequence. Result: The secondary structure of the stems of A. hexapetalus consisted of the periderm

cortex and vascular cylinder (stele). Sieve and tubes phloem fibers

scattering in bundles

arranged at intervals. The leaves of A. hexapetalus were of typically bifacial

consisted of epidermis

mesophyll and veins. The midrib vascular bundle

collateral vascular bundle

was ring with vascular bundle sheath. Endophytic fungi existed in the local cells of cortex

phloem

palisade tissue and spongy tissue. A total of 18 strains was isolated and identified

among them Phomopsis spp. accounted for 61.1% in all strains of endophytic fungi. Conclusion: The microscopic characteristics of A. hexapetalus's stem and leaf can be used as a reference for identification. The endophytic fungi of A. hexapetalus were distributed with no obvious tissue specifity and P. spp. were determined as dominant species.