Objective:To evaluate dynamic accumulation of active ingredient in different part and growth phase of caltivative Zanthoxylum nitidum

and provide the basis for the GAP planting and the rational utilization. Method: A sensitive and accurate method was developed to ditermined the nitidine chloride in different parts (root

stem

and branch) and different harvest times of Z. nitidum by ultra performance liquid chromatography (UPLC) with diode array detector (DAD) for the first time. Result: The variation of the nitidine chloride contents was existed in different parts of Z. nitidum

which sequence was showed as follows:root>stem>branch

there was regulation dynamic changes of nitidine chloride content in different part and growth phase

which exist two obvious peak and a valley

that is the content of nitidine chloride reached highest value at May and October and lowest at June. Conclusion: The active ingredient distributed in caltivative Z. nitidum was different in different part and growth phase

the optimal harvest time was at October and November in the third-year

and the root was the main medicinal parts. All part of Z. nitidum have the medicinal value.