Objective:To investigate the major effective compositions in Jianpi Huoxue decoction against lipid peroxidation which can protect alcohol-induced liver injury remarkably and its possible action mechanism. Method: The rats were given Lieber-DeCarli liquid diet daily to make the alcoholic liver injury model. And Uniform design was adopted to design the experiments. malonyldialdehyde(MDA) in liver tissue was detected as the index. The same mode was used to screen the major herbs and verify the efficacy. The superoxide dismutase(SOD)

nitric oxide synthase(NOS)

induced nitric oxide synthase(iNOS)

glutathione peroxidase(GSH-Px)in liver and Fe2+ in serum were detected. Result: Puerariae Lobatae Radix

Alismatis Rhizoma

Schisandrae Chinensis Fructus

and turmeric were the major herbs in Jianpi Huoxue decoction against lipid peroxidation. And the verification experiment showed that compared with the model group

the major herbs could reduce the MDA

NOS

iNOS and Fe2+ significantly and promote the activity of SOD

GSH-Px apparently. Conclusion: The combination of Puerariae Radix

Alismatis Rhizoma

Schisandrae Chinensis Fructus

and turmeric were the major effective compositionsof Jianpi Huoxue decoction against lipid peroxidation. The possible action mechanism may lie in inhibiting NOS

iNOS and Fe2+ in serum which promote oxidation

and increase the activity of free radical scavenger SOD

GSH-Px which provide anti-oxidation.