Objective:To observe the expression of core binding factor alpha 1 mRNA of femoral organization by Zuogui Pill in ovariectomy-induced osteoporosis rats. Method: Two hundred and eighty male SPF SD rats were divided into three groups:normal group 40;sham-operated group 40;ovariectomy-induced osteoporosis model was adopted

21 days later

randomly modeling rats were divided into 5 groups:the model group

nilestriol group

Zuogui Pill high

medium

low dose group. Zuogui Pill groups were ig given with Zuogui Pill at dosage of 6.4

3.2

1.6 g·kg-1

once per day. Nilestriol group was treated with Nilestriol liquid. The normal group

sham-operated group and the model group were given with distilled water.60

120

180 d later

the expression of core binding factor alpha 1 in the femoral organization was determined by RT-polymerase chain reaction (PCR). Result: Compared with the normal group

in the model group the femoral core binding factor alpha 1 mRNA level obviously reduced (P<0.01). And compared with the model group

Zuogui Pill groups increased femoral core binding factor alpha 1 mRNA level significantly(P<0.05). Conclusion: The down-regulated expression of core binding factor alpha 1 mRNA of bone tissue may be contributed to PMOP

Zuigui Pill can up-regulated mRNA expression.