Objective:To observe the impact of Qingchang Huashi formula(QHF) on mouse bone marrow-derived dendritic cell(DC) antigen-presenting function and explore the mechanism of action of the treatment of UC. Method: The DCs ftransfected by nuclear factor κB decoy oligonucleotides(NF-κB ODN) were as control group to observe the change the characters of the DC after incubation of QHF

and then to observe whether QHF can influence the function of DC antigen-presenting by inhibiting the expression of NF-κB. The experiment was divided into six groups

which is blank group

QHF group

ODN transfection group

QHF and lipopolysaccharides(LPS) group

ODN transfection and LPS group. Flow cytometry was used to detect the DC surface of CD11c

CD40

MHC II expression and immune fluorescence was used to detect the nuclear translocation of NF-κB of each group. Result: QHF could effective ly reduce the DC surface antigens CD40 and MHC II costimulatory molecule expression

and inhibit NF-κB activation into the nucleus. Conclusion: By inhibiting the expression of NF-κB

affecting maturation and differentiation of DC

reducing the antigen-presenting function

thereby reducing the inflammatory response are the main mechanism of QHF treating UC.