Objective:To study the effects of baicalin on the proliferation

invasion and migration of A549 cells

and tube formation of Human umbilical vein endothelial cells (HUVEC)on matrigel in vitro

as well as the antitumor effect of baicalin on A549 cell xenografts in vivo. Method: A549 cells were treated by baicalin within different concentrations (25

50

100

200

400 μmol·L-1)24

48

72 h respectively

the cell density was 1×104/mL

cell proliferation was validated by Thiazolyl blue terazolium bromide (MTT) method. The changes of cell invasion and migration in vitro were detected by Transwell assay

the anti-angiogenic effect was detected by tube formation experiment

the concentration of baicalin of the three experiments were 12.5

25

50 μmol·L-1. Antitumor efficacy of baicalin was evaluated in nude mice models of human lung cancer xenograft

the nude mice were divided into 4 groups

the high dose group of baicalin (100 mg·kg-1)

the low dose group of baicalin (50 mg·kg-1)

the solvent control group and the cisplatin(1 mg·kg-1) group. The administration method of the three former groups was by gavage for 21 days consecutively

the cisplatin group was by intraperitoneal injection for 10 days consecutively. Result: ①After treated with different concentrations of baicalin for 24

48

72 hour

the growth of A549 cells were significantly inhibited(P<0.05 to P<0.01)

the IC50of baicalin in different times were 100.77

75.02

55.81 μmol·L-1 respectively. ② Baicalin(25

50 μmol·L-1) could inhibit the invasion and migration of A549 cells

therefore

it inhibit the metastatic of cancer cells. ③The tubule structure formation treated with baicalin(25

50 μmol·L-1) was reduced compared with the control group. ④ Baicalin(50 mg·kg-1) could inhibit the A549 cell xenograft in vivo. Conclusion: These results suggest that baicalin could inhibit the proliferation

invasion and migration of A549 cells and the tube formation of Human umbilical vein endothelial cells in vitro

in vivo baicalin could inhibit the A549 cell xenograft

its mechanism on lung cancer need further study.