Objective:To observe the effect of total alkaloids from Phyllodium pulchellum on proliferation of human hepatic stellate cell line Lx-2 and the impact on secretion of collagen and cytokines which are related to liver fibrosis. Method: After recovery

Lx-2 cells in logarithmic growth were diluted to a concentration of 1×105/mL

and inoculated 0.1 mL in hole in incubator at 37 ℃

blank control group was set up

the concentration of the drug administered in an order of 100

75

55

40

30

20

15

10 mg·L-1. After 48 h of administration

MTT assay was used to detect cell proliferation. Similarly

after recovery

Lx-2 cells in logarithmic growth were diluted to a concentration of 1×105/mL

and inoculated 0.1 mL/hole in incubator at 37 ℃

blank control group and Colchicine group (6.25 mg·L-1) were set up. After 48 h of administration

ELISA method was used to detect Lx-2 cell supernatant for type I collagen (COL I)

type III collagen (COL III)

type IV collagen (COL Ⅳ)

transforming growth factor β1(TGF β1) and platelet-derived growth factor (PDGF) content. Result: Compared with the control group

Lx-2 cell proliferation was inhibited by the total alkaloids in a concentration-dependent manner with inhibition rates of 49.4%

25.9%

24.7%

15.3%

15.3%

12.9%

2.35% and 1.18% accordingly. Compared with the control group

the total alkaloids of 40 mg·L-1 could significantly inhibit COL I

COL Ⅲ

COL Ⅳ

TGF-β1 and PDGF secretion ( P<0.05 or P <0.01). Conclusion: The total alkaloids from P. pulchellum can inhibit the proliferation of Lx-2 cells and the secretion of COLⅠ

COL III

COL IV

TGF-β1and PDGF.