Objective: To establish HPLC fingerprint detection for alkaloids from extract of Sophora flavescens in Yuchangning capsules. Method: Amino Kromasil NH2 column (4.6 mm×250 mm

5 μm) was adopted

gradient eluted with mobile phase of acetonitrile-ethanol (8:1)-3% phosphoric acid water

flow rate was 0.8 min·mL-1

detection wavelength was 220 nm.10 batches of extract of S. flavescens were detected by HPLC fingerprint

then evaluated with 2004 edition of ‘chromatographic fingerprint evaluation system of Chinese medicine’. Result: By taking Kromasil NH2 column

peaks separation of extract of S. flavescens was better

identified 15 common peaks and 4 of them were assigned. Similarity of these 10 batches extract was more than 0.9

correlation between fingerprints of extract and raw materials existed. Conclusion: This method had good precision

repeatability and stability

it could provide a detection method for quality control of extract of S. flavescens.