铁皮石斛中总氨基酸的含量测定及提取工艺优选

王培培; 鲁芹飞; 李婧; 黄松; 陈建南

doi:10.11653/syfj2013140030

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铁皮石斛中总氨基酸的含量测定及提取工艺优选

更新时间：2024-01-04

- 铁皮石斛中总氨基酸的含量测定及提取工艺优选
- Optimization of Ultrasonic Extraction Technology and Content Determination of Total Amino Acids from
- 中国实验方剂学杂志 2013年19卷第14期页码：30-33
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"十二五"科技支撑计划课题(2011BAI01B02);广东省科技厅中药现代化专项(K5080016);广东省科技计划项目(2008A030101009);广东省科技计划项目(2011A030100011);广东省教育部产学研结合项目(2010B090400474)
- DOI：10.11653/syfj2013140030
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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王培培, 鲁芹飞, 李婧, 等. 铁皮石斛中总氨基酸的含量测定及提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(14):30-33.

WANG Pei-pei, LU Qin-fei, LI Jing, et al. Optimization of Ultrasonic Extraction Technology and Content Determination of Total Amino Acids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 30-33.
王培培, 鲁芹飞, 李婧, 等. 铁皮石斛中总氨基酸的含量测定及提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(14):30-33. DOI： 10.11653/syfj2013140030.

WANG Pei-pei, LU Qin-fei, LI Jing, et al. Optimization of Ultrasonic Extraction Technology and Content Determination of Total Amino Acids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 30-33. DOI： 10.11653/syfj2013140030.

摘要

目的: 优选铁皮石斛中总氨基酸提取工艺

并建立其含量测定方法。方法: 以天冬氨酸为对照品

采用UV测定铁皮石斛中总氨基酸含量;在单因素试验基础上

采用L9(34)正交试验考察提取时间、提取次数及溶剂用量对铁皮石斛中总氨基酸提取工艺的影响。结果: 最佳提取工艺为加20倍量0.1 mol·L-1 HCl超声提取30 min

提取1次。结论: 优选的提取工艺稳定可行

建立的含量测定方法简便、准确、重复性好。

Abstract

Objective: To optimize extraction technology of total amino acids from Dendrobium officinale and establish its content determination method. Method: The content of total amino acids was determined by UV with aspartic acid as reference;Effects of the concentration of extraction solvent

solid-liquid ratio

extraction temperature

extraction time and times on yield of total amino acids were investigated by single factor tests

basis on these

extraction technology was optimized by orthogonal test. Result: Optimum ultrasonic extraction conditions were as follow:extracted 1 time at 60℃ with 20 times the amount of 0.1 mol·L-1 HCl for 30 min. Conclusion: This optimized technology was stable and feasible

established method of content determination was convenient and accurate with good repeatability.

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