Objective: To establish quality standards of Shengxue Xiaoban tablets. Method: TLC was adopted to identify Indigo Naturalis

Forsythiae Fructus and Moutan Cortex.Indirubin was as quantitative indicroe

whose content was determined by RP-HPLC

chromatography conditions were as follows: Discovery C18 column(4.6 mm×200 mm

5 μm)

mobile phase of methanol-0.1 mol·L-1 ammonium acetate solution-acetic acid (80:20:1)

detection wavelength 290 nm. Result: TLC identification showed that negative controls were no interference;Linear range of indirubin was 0.010 95-0.394 2 μg(r=0.999 8)

average recovery was 97.4% with RSD of 0.7%. Conclusion: This established method was simple and accurate which could be used to quality control of Shengxue Xiaoban tablets.