Objective: Using the HPLC and atomic fluorescence spectrometry

to analyze notoginsenoside R1

ginsenoside Rg1

ginsenoside Rb1 and the total arsenic content in Panax notoginseng and its two extracting solutions. Method: The chromatographic separation was achieved on an Agilent TC-C18(4.6 mm×250 mm

5 μm) column with acetonitrile (A)-0.05% phosphoric acid (B) as mobile phase for gradient elution at the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the detection wavelength was set at 203 nm. Treat Panax notoginseng with different extracting manners (unprocessed

traditional decocting and refluxing by ethanol). Run wet digestion process before measuring the fluorescence intensity

and then get the content of total arsenic. Result: The content of total arsenic differs in different extracting manner

unprocessed>traditional decocting>refluxing by ethanol. Conclusion: Panax notoginseng refluxing by ethanol can extract more pharmaceutical components with low total arsenic. The traditional decocting could be replaced by the ethanol way.