RP-HPLC同时测定韭菜子中尿苷、腺苷的含量

盛康美; 杨立新; 赵海誉; 司南; 杨健; 王宏洁

doi:10.11653/syfj2013140152

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RP-HPLC同时测定韭菜子中尿苷、腺苷的含量

更新时间：2024-01-04

- RP-HPLC同时测定韭菜子中尿苷、腺苷的含量
- Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC
- 中国实验方剂学杂志 2013年19卷第14期页码：152-154
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- DOI：10.11653/syfj2013140152
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- 纸质出版日期：2013
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盛康美, 杨立新, 赵海誉, 等. RP-HPLC同时测定韭菜子中尿苷、腺苷的含量[J]. 中国实验方剂学杂志, 2013,19(14):152-154.

SHENG Kang-mei, YANG Li-xin, ZHAO Hai-yu, et al. Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 152-154.
盛康美, 杨立新, 赵海誉, 等. RP-HPLC同时测定韭菜子中尿苷、腺苷的含量[J]. 中国实验方剂学杂志, 2013,19(14):152-154. DOI： 10.11653/syfj2013140152.

SHENG Kang-mei, YANG Li-xin, ZHAO Hai-yu, et al. Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 152-154. DOI： 10.11653/syfj2013140152.

摘要

目的: 建立同时测定韭菜子中的尿苷和腺苷的RP-HPLC含量测定方法。方法: CNW C18-WP色谱柱(4.6 mm×250 mm

5 μm)

流动相为A(水)-B(乙腈)

梯度洗脱(0~20 min

100%~80%A

0%~20%B);检测波长260 nm。结果: 尿苷和腺苷分别在9.56~95.60 ng和10.20~102.0 ng与峰面积呈良好线性关系

r分别为0.999 7

0.999 6;平均回收率分别为98.04%

101.99%

RSD分别为0.99%

1.44%。结论: 该方法简便易行

具有良好的重复性和稳定性

可用于韭菜子质量控制。

Abstract

Objective: To establish the RP-HPLC quantitative determination method of uridine and adenosine in Allii Tuberosi Semen. Method: CNW C18-WP column (4.6 mm×250 mm

5 μm) was applied with water (A)-acetonitrile (B) as mobile phase. The gradient elution condition was 0-20 min

0%-20% B and the detection wavelength was set at 260 nm. Result: Uridine and adenosine revealed good linear relation from 9.56 to 95.60 ng and from 10.20 to 102.0 ng respectively with correlation coefficient (r) of 0.999 7 and 0.999 6.The average recoveries of them were 98.04% and 101.99% with RSD of 0.99% and 1.44%. Conclusion: This method was simple and accurate with good repeatability and stability

which was suitable for the quality control of Allii Tuberosi Semen.

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