Objective: To study the effects of Guifu Lizhong Wan on hippocampus aging in mice deficiency of kidney and spleen model. Method: Forty SD male mice were randomly divided into normal control group

model group

vitamin E group

Guifu Lizhong Wan group

10 each group. The normal control group was im treated with 5 mL·kg-1saline and ig with 10 mL·kg-1 at 8:00 am

and 2:00 pm

respectively. The other groups were injected hydrocortisone with 25 mg·kg-1and liquid of Rheum palmatum decoction were administered with 4 g·kg-1at 8:00 am

the model group was administered saline with 10 mL·kg-1

vitamin E group was administered vitamin E with 18×10-3g·kg-1 and Guifu Lizhong Wan groups were given Guifu Lizhong Wan with 1.62 g·kg-1 at 2:00 pm

once a day 30 days. Observation of ultrastructure of hippocampus changes; testing the brain hippocampus cells cycles by flow cytometry. Result: In Hippocampus the highest proportion of G1 phase(P<0.05) was found

compared with the model control group

the vitamin E and Guifu Lizhong Wan increased organization organelles with fewer neuron atrophy

and neurons synaptic gap widening

meanwhile the cell aging rate decreased obviously(P<0.05). Conclusion: Guifu Lizhong Wan has an effect on hippocampus aging in mice deficiency of kidney and spleen model