Objective: To investigate the neuroprotective effect of Shengmai injection (SMI) on the expression of tissue factor (TF) and related signal pathways in mice with cerebral ischemia-reperfusion injury. Method: Mice were randomly divided into 5 groups as following:sham operation group

ischemia-reperfusion(I/R) group

SMI groups at three doses (5.68

2.84

1.42 g·kg-1). Right middle cerebral artery was occluded by inserting a thread through internal carotid artery for 1 h. The infarct volume and neurological scores were evaluated after 24 h reperfusion. Western blotting was used to determine the expression of tissue factor (TF)

nuclear factor-kappa B (NF-κB) subunit p65

Akt

and their phosphorylation after 1 h reperfusion. Result: Compared with I/R group

SMI could significantly reduce the infarct volume and improve the neurological deficits in a dose-dependent manner. SMI also remarkably down-regulated the expression of TF

inhibited the activation of NF-κB induced by ischemia-reperfusion and the phosphorylation of Akt in mice. Conclusion: Shengmai injection

at certain dosage range

elicits potent protection against cerebral ischemia/reperfusion injury

which may partly be due to inhibiting TF expression and its related pathways.