Objective: To evaluate the toxicity effect of raw Radix Aconiti and raw Rhizoma Pinelliae on lactation rat's myocardial cells and the relationship between the toxicity effect and different proportion. Method: The SD lactation rat's myocardial cells were separated and cultivated when born in 3 days. The four groups were raw Radix Aconite

Radix Aconiti and raw Rhizoma Pinelliae 1:0.25

1:0.5

1:1(Ⅰ

Ⅱ

Ⅲ

Ⅳ)were given the final concentration of the Radix Aconiti of 0

0.016

0.078

0.235

0.392

0.548

0.705

0.861

1.018 g·mL-1·L-1] to the myocardiaL cells

respectively cultivating 1

2

4

12 h

to observe the cell pulsation frequency in different time

with MTT method to detect the percentage of the cell survival at 12 hours

and calculate half inhibition concentration (IC50)by probability unit method. Result: ①At 12 h

the inhibition rate (CI) of raw Radix Aconiti group was significantly higher than other groups (P<0.01)

and with the increasing of theproportion of the raw pinellia tuber

the inhibition rate was decreased. ② The IC50 ofⅠ

Ⅱ

Ⅲ

Ⅳ group were 0.446

0.460

0.530

0.575 g·mL-1 ③ When the final concentration of raw Radix Aconiti is 0.016-0.078 g·mL-1

the cell pulsation frequency was increased in 1-2 h

and reduced in 4-12 h. Between 0.235-0.861 g·mL-1

the cell pulsation frequency was reduced in 1 h

and rised when 2 h

but less than normal level

then falled in 4-12 h.Dose with 1.018 g·mL-1

the cells were immediately arrested. Conclusion: The addition of Raw pinellia reduced the toxicity of the raw Radix Aconiti

and there is a significant dose-response relationship.