Objective: To study the effect of Chinese herbal compound extract on cigarette mainstream smoke induced damage. Method: The effect of cigarette mainstream smoke on cytotoxicity toward Chinese hamster ovarian cells(CHO cells) was detected.Salmonella mutagenicity test

micronucleus test

proliferation of human bronchial epithelial cell line (BEP2D) cells in vitro were also employed

peripheral blood cells

ammonia induced cough

phenolsulfanphthalein excretion test in vivo were carried out after animals were exposed to cigarette smoke for 30 days. Intervention effects of Chinese herb compound on all these tests were evaluated after added in cigarette at 0.03%. Result: After added in cigarette at 0.03%

Chinese herbal compound extract did not increase thesalmonella mutagenicity and micronucleus frequency and could reduce the cytotoxicity toward CHO cells induced by cigarette smoke. Chinese herbal compound extract could reduce the injury of human respiratory epithelial cells. For the research that animals were exposed to cigarette smoke for 30 days

Chinese herbal compound extract (2 cigarette·d-1) could inhibit the decrease in hemoglobin (P<0.05);4 cigarette·d-1could inhibit the increase in platelet count and the increasing of times of cough (p<0.05);8 cigarette·d-1 could reverse the shortenedlatent period of cough (P<0.05)

and reverse the decrease of excretion of phenolsulfonphthalein (P<0.05). Conclusion: Chinese herbal compound extract doesn't increase the in vitro cytotoxicity of cigarette smoke and even shows reducing effect or tendency

Chinese herbal compound extract could reduce the injury of cigarette smoke by reducing the cough

thick sputum and change in peripheral blood cells induced by smoking

and though directly protective effect on respiratory epithelial cells.