Objective: To study the protective effect of the total flavonoids of bidens (TFB) on the D-GalN induced acute liver injury in mice. Method: Acute liver injury in mice was established by intraperitoneal injection of D-GalN. The mice were randomly divided into five groups:model group

bifendate group (120 mg·kg-1)

low

medium

high-dosage groups of TFB (60

120

240 mg·kg-1). A normal control group was set up also. The index of thymus

spleen and liver were measured. The serum levels of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) and alkaline phosphatase (AKP)

serum contents of albumin (Alb) and total antioxidant capacity (T-AOC) were examined using colorimetric detection. And the expression of inducibility nitric oxide synthase (iNOS) in liver tissue was determined by western blot analysis. Pathomorphological changes in microscopic slodes were observed by hematoxylin-eosin staining (HE). Result: Compared to model group

TFB markedly increased the index of thymus

spleen

and liver (P<0.01)

and lowered the serum levels of ALT

AST and AKP (P<0.01)

while elevated the contents of Alb

T-AOC (P<0.01)

down-regulated the expression of iNOS (P<0.01)

and alleviated the liver damage condition. Conclusion: The total flavonoids of bidens has protective effect on the D-GalN induced liver injury in mice. The mechanism may be related to antioxidation and inhibition of cytotoxicity.