Objective: To compare stability and reproducibility of three preparation processes for Hypericum perforatum extract. Method: HPLC fingerprint of H. perforatum extract was established

and different batches of extract were analyzed by similarity analysis

cluster analysis and pattern recognition. Result: These three processes had good stability and reproducibility

and enzymatic hydrolysis was superior to before enzymatic hydrolysis

purification was superior to before purification

optimum preparation technology was purified by LSA-10 macroporous resin after enzymatic hydrolusis.Mirror degree of different batches of extract was high with good similarity(>0.9). Conclusion: HPLC fingerprint analysis method could be adopted for quality control and process evaluation of similar extract.