素馨花总环烯醚萜苷的大孔树脂富集纯化工艺优选

罗赛赛; 郝婷; 赵桂琴

doi:10.11653/syfj2013150013

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素馨花总环烯醚萜苷的大孔树脂富集纯化工艺优选

更新时间：2024-01-04

- 素馨花总环烯醚萜苷的大孔树脂富集纯化工艺优选
- Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from by Macroporous Resin
- 中国实验方剂学杂志 2013年19卷第15期页码：13-16
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省自然科学基金项目(C2010001354)
- DOI：10.11653/syfj2013150013
  中图分类号：
- 纸质出版日期：2013
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罗赛赛, 郝婷, 赵桂琴. 素馨花总环烯醚萜苷的大孔树脂富集纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(15):13-16.

LUO Sai-sai, HAO Ting, ZHAO Gui-qin. Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 13-16.
罗赛赛, 郝婷, 赵桂琴. 素馨花总环烯醚萜苷的大孔树脂富集纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(15):13-16. DOI： 10.11653/syfj2013150013.

LUO Sai-sai, HAO Ting, ZHAO Gui-qin. Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 13-16. DOI： 10.11653/syfj2013150013.

摘要

目的: 优选大孔树脂富集纯化素馨花总环烯醚萜苷的工艺条件。方法: 采用HPLC测定橄榄苦苷含量。以橄榄苦苷为指标成分

通过静态吸附-洗脱试验筛选大孔树脂型号

通过单因素试验和正交试验考察大孔树脂富集纯化工艺。结果: XDA-16型大孔树脂吸附洗脱性能最好

最佳纯化工艺条件为上样液生药质量浓度0.08 g·mL-1

上样量0.7 g·g-1

树脂柱径高比1:3

吸附流速2 BV·h-1

加8 BV水洗除杂

用70%乙醇4 BV于2 BV·h-1流速洗脱

收集洗脱液。结论: 该工艺条件稳定可行

XDA-16型大孔树脂可有效地富集纯化素馨花总环烯醚萜苷。

Abstract

Objective: To optimize enrichment and purification technology of total iridoid glycosides from Jasminum officinale by macroporous resin. Method: With oleuropein as index

its content was determined by HPLC. Types of macroporous resin were optimized by static adsorption-elution test

enrichment and purification technology was investigated by orthogonal test and single-factor tests. Result: XDA-16 macroporous resin showed better effect than others

optimum purification technology was as following:the concentration of sample liquid 0.08 g·mL-1

height-diameter ratio of 1:3

absorption velocity 2 BV·h-1

dosage of sample liquid of 0.7 g·g-1

washed impurity with 8 BV water

then eluted wtih 4 BV 70% ethanol at 2 BV·h-1

collected eluent. Conclusion: This optimized technology was stable and feasible.XDA-16 macroporous resin had a good enrichment and purification effect on total iridoid glycosides from J. officinale.

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