洋金花中醉茄内酯总甾体的纯化工艺优选

王艳艳; 孙海姣; 王知斌; 王秋红; 杨炳友; 匡海学

doi:10.11653/syfj2013150017

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洋金花中醉茄内酯总甾体的纯化工艺优选

更新时间：2024-01-04

- 洋金花中醉茄内酯总甾体的纯化工艺优选
- Optimization of Purification Technology for Total Steroids of Withanolides from Effective Part of
- 中国实验方剂学杂志 2013年19卷第15期页码：17-19
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30371736,30672633);国家科技重大专项"重大新药创制"项目(2009ZX09102-117);全国优秀博士论文作者专项资金项目(200980);哈尔滨市科技攻关计划项目(2012DB3BS055)
- DOI：10.11653/syfj2013150017
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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王艳艳, 孙海姣, 王知斌, 等. 洋金花中醉茄内酯总甾体的纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(15):17-19.

WANG Yan-yan, SUN Hai-jiao, WANG Zhi-bin, et al. Optimization of Purification Technology for Total Steroids of Withanolides from Effective Part of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 17-19.
王艳艳, 孙海姣, 王知斌, 等. 洋金花中醉茄内酯总甾体的纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(15):17-19. DOI： 10.11653/syfj2013150017.

WANG Yan-yan, SUN Hai-jiao, WANG Zhi-bin, et al. Optimization of Purification Technology for Total Steroids of Withanolides from Effective Part of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 17-19. DOI： 10.11653/syfj2013150017.

摘要

目的: 优选洋金花中醉茄内酯总甾体的大孔吸附树脂纯化工艺。方法: 以醉茄内酯总甾体含量和纯度为指标

通过比较不同型号大孔树脂对甾体成分的吸附性能和解析能力

筛选树脂类型。采用单因素试验考察上样量、树脂量、上样速度、洗脱剂种类、洗脱速度、用量等对纯化工艺的影响。结果: 选用D941型大孔树脂

其最佳纯化工艺为上样量-树脂量<7.5 g·L-1

上样液pH 7

加3 BV水以6 BV·h-1流速洗脱

总甾体的纯度>90%。结论: 该优选工艺简单可行

纯化效果好。

Abstract

Objective: To optimize separation and purification technology for total steroids of withanolides from effective part of trearment psoriasis in Datura metel. Method: With content and purity of total steroids as indexes

optimum resin model was screened by comparing adsorption properties and elution ability of different macroporous resins.Single factor tests were adopted to investigate effect of loading capacity

type of eluent

elution rate and other parameters on purification technology. Result: D941 macroporous resin was selected

its optimum purification technology was as following:ratio of sample mass to macroporous resin<7.5 g·L-1

pH of sample liquid 7

eluted with 3 BV water at elution flow speed of 6 BV·h-1.After purified

purity of total steroids was more than 90%. Conclusion: This optimized purification technology was simple

stable and feasible with good purification effect.

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