Objective: To optimize smashing tissue extraction technology of total naphthaquinones in Arnebia euchroma. Method: With transfer rates of total naphthaquinones and β

β'-dimethylacrylalkannin as evaluation indexes

L9(34) orthogonal design was adopted for investigating effect of the amount of solvent

extraction time and voltage on smashing tissue extraction technology

and compared with percolation method. Result: Optimum extraction conditions were as follows:extracted 2 times with 14

8 folds the amount of 95%ethanol at 140 V

2 minutes per time.Transfer rates of total naphthaquinones and β

β'-dimethylacrylalkannin were 90.72% and 70.68%

but they were 79.03% and 65.43% with percolation method

respectively. Conclusion: Smashing tissue extraction method was better than percolation method

the former was suitable for extracting total naphthaquinones in A. euchroma

this optimized technology was stable and feasible with good application prospects.