Objective: To comparative analyze different extraction methods for protein in tissue of Saussurea involucrate. Method: Protein in tissue of S. involucrate was extracted by phenol method

TCA/acetone method and urea method

prepared protein was detected by one-dimensional SDS-PAGE and two-dimensional electrophoresis

then optimum extraction method for protein in tissue of S. involucrate was determined by comparing electrophoretic patterns. Result: Phenol extraction method was optimal with the highest purity and the least impurities

one-dimensional SDS-PAGE and two-dimensional electrophoresis showed that there were more protein spots and species. Conclusion: Three extraction methods had some protein loss

but phenol method was the best for extracting protein from tissue of S. involucrate

this study could provide reference for investigating proteomics of S. involucrate.