Objective: To develop a phosphomolybdic acid-casein spectrophotometry for determination of total tannins in fruits of Phyllanthus emblica from different areas. Method: With gallic acid as reference substance and phosphomolybdic acid as chromogenic agent

detection wavelength 760 nm

the content of total tannins in P. emblica from eight different areas was determined. Result: The linearity range of gallic acid was 0.306-2.448 mg·L-1 with r=0.999 1

the average recovery was 99.27% with RSD 2.30%.The content of total tannins in P. emblica from different areas were significantly different (6.72%-11.95%)

the content of total tannins in samples from India and Nepal were the highest. Conclusion: This method was simple

accurate and reproducible which could be adopted for determination of total tannins in P. emblica.