HPLC测定穿心莲配伍绵茵陈后2种内酯成分含量的变化

陈倩倩; 李清; 齐会红; 李玉娇; 毕开顺

doi:10.11653/syfj2013150064

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HPLC测定穿心莲配伍绵茵陈后2种内酯成分含量的变化

更新时间：2024-01-04

- HPLC测定穿心莲配伍绵茵陈后2种内酯成分含量的变化
- Determination of Content Change of Two Lactone Ingredients from Andrographis Herba after Compatibility with Artemisiae Scopariae Herba by HPLC
- 中国实验方剂学杂志 2013年19卷第15期页码：64-66
- 作者机构：
- 作者简介：
- 基金信息：
  
  2012年辽宁省高等学校中药质量控制关键技术创新团队项目(LT2012018)
- DOI：10.11653/syfj2013150064
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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陈倩倩, 李清, 齐会红, 等. HPLC测定穿心莲配伍绵茵陈后2种内酯成分含量的变化[J]. 中国实验方剂学杂志, 2013,19(15):64-66.

CHEN Qian-qian, LI Qing, QI Hui-hong, et al. Determination of Content Change of Two Lactone Ingredients from Andrographis Herba after Compatibility with Artemisiae Scopariae Herba by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 64-66.
陈倩倩, 李清, 齐会红, 等. HPLC测定穿心莲配伍绵茵陈后2种内酯成分含量的变化[J]. 中国实验方剂学杂志, 2013,19(15):64-66. DOI： 10.11653/syfj2013150064.

CHEN Qian-qian, LI Qing, QI Hui-hong, et al. Determination of Content Change of Two Lactone Ingredients from Andrographis Herba after Compatibility with Artemisiae Scopariae Herba by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 64-66. DOI： 10.11653/syfj2013150064.

摘要

目的: 考察穿心莲与绵茵陈配伍后对穿心莲内酯和脱水穿心莲内酯含量变化的影响。方法: 采用RP-HPLC

Innersil ODS-3色谱柱(4.6 mm×250 mm

5.0 μm)

流动相乙腈-水梯度洗脱

流速1.2 mL·min-1

波长检测程序(0~10 min

225 nm;10~20 min

254 nm)

柱温35℃。结果: 穿心莲内酯和脱水穿心莲内酯质量浓度分别在19.66~314.6(r=0.999 5)

4.32~69.12 g·L-1(r=0.999 5)与峰面积呈良好线性关系

平均加样回收率分别为99.7%

99.9%

RSD均<2.3%。结论: 穿心莲与绵茵陈配伍后水煎液中2种内酯类成分含量显著增加。

Abstract

Objective: To investigate effects of Andrographis Herba compatibility with Artemisiae Scopariae Herba on content change of andrographolide and dehydroandrographolide. Method: RP-HPLC was performed on an Innersil ODS-3 column (4.6 mm×250 mm

5.0 μm) with mobile phase of acetonitrile-water in gradient elution

flow rate was 1.2 mL·min-1

detection wavelengths were set at 225 nm for andrographolide in 0-10 min and 254 nm for dehydroandrographolide in 10-20 min

column temperature was 35℃. Result: The linear range of andrographolide and dehydroandrographolide were 19.66-314.6(r=0.999 5)

4.3-69.12 g·L-1(r=0.999 5)

respectively.Average recoveries of these two components were 99.7% and 99.9%

respectively with RSD were less than 2.3%. Conclusion: The contents of andrographolide and dehydroandrographolide from Andrographis Herba increased significantly after compatibility with Artemisiae Scopariae Herba.

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