HPLC 同时测定林蛙油中-亚麻酸、亚油酸和油酸含量

于桂芳; 秦建平; 李家春; 王婧; 毕宇安; 王振中; 萧伟

doi:10.11653/syfj2013150082

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HPLC 同时测定林蛙油中-亚麻酸、亚油酸和油酸含量

更新时间：2024-01-04

- HPLC 同时测定林蛙油中-亚麻酸、亚油酸和油酸含量
- Determination of -Linolenic Acid, Linoleic Acid and Oleic Acid in Oviductus Ranae by HPLC
- 中国实验方剂学杂志 2013年19卷第15期页码：82-85
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部973计划项目(2010CB735604)
- DOI：10.11653/syfj2013150082
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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于桂芳, 秦建平, 李家春, 等. HPLC 同时测定林蛙油中-亚麻酸、亚油酸和油酸含量[J]. 中国实验方剂学杂志, 2013,19(15):82-85.

YU Gui-fang, QIN Jian-ping, LI Jia-chun, et al. Determination of -Linolenic Acid, Linoleic Acid and Oleic Acid in Oviductus Ranae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 82-85.
于桂芳, 秦建平, 李家春, 等. HPLC 同时测定林蛙油中-亚麻酸、亚油酸和油酸含量[J]. 中国实验方剂学杂志, 2013,19(15):82-85. DOI： 10.11653/syfj2013150082.

YU Gui-fang, QIN Jian-ping, LI Jia-chun, et al. Determination of -Linolenic Acid, Linoleic Acid and Oleic Acid in Oviductus Ranae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 82-85. DOI： 10.11653/syfj2013150082.

摘要

目的: 建立同时测定林蛙油中α-亚麻酸、亚油酸和油酸含量的HPLC方法。方法: Alltima C18色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-0.1%磷酸(80:20)为流动相

流速1 mL·min-1

柱温30℃

检测波长203 nm。结果: α-亚麻酸在4.76~152.16 mg·L-1 (r=1)

亚油酸在15.36~491.59 mg·L-1 (r=0.999 8)

油酸在47.28~1512.80 mg·L-1 (r =0.999 9) 呈良好的线性关系;平均回收率α-亚麻酸为95.8%

RSD 1.79% (n=6);亚油酸为98.6%

RSD 1.32% (n=6);油酸为99.3%

RSD 1.59% (n=6)。结论: 该法操作简便

结果准确

专属性强

重复性好

可作为林蛙油质量控制的参考。

Abstract

Objective: To establish a HPLC method for the determination of α-linolenic acid

linoleic acid and oleic acid in Oviductus Ranae. Method: Alltima C18(4.6 mm× 250 mm

5 μm) column was used with mobile phase of acetonitrile-0.1% phosphoric acid (80:20);the flow rate was 1 mL·min-1;the column temperature was kept at 30℃;the detection wavelength was set at 203 nm. Result: The linear ranges of α-linolenic acid

linoleic acid and oleic acid were 4.755-152.16 mg·L-1 (r=1)

15.362-491.59 mg·L-1 (r=0.999 8) and 47.275-1512.8 mg·L-1 (r=0.999 9) respectively.The average recoveries were 95.8% with RSD 1.79% for α-linolenic acid

98.6% with RSD 1.32% for linoleic acid

99.3% with RSD 1.59% for oleic acid. Conclusion: The method is simple

accurate

convenient

specific and repeatable

which can be used to control the quality of α-linolenic acid

linoleic acid and oleic acid in Oviductus Ranae.

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