UPLC同时测定银黄颗粒中4种黄酮类成分含量

乔东鸽; 吉丽娜; 何希荣; 冯伟红; 李东影

doi:10.11653/syfj2013150102

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UPLC同时测定银黄颗粒中4种黄酮类成分含量

更新时间：2024-01-04

- UPLC同时测定银黄颗粒中4种黄酮类成分含量
- Simultaneous Determination of Four Flavones in Yinhuang Granule by UPLC
- 中国实验方剂学杂志 2013年19卷第15期页码：102-105
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"重大新药创制"科技重大专项(2009ZX09301-005,2009ZX09308-003)
- DOI：10.11653/syfj2013150102
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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乔东鸽, 吉丽娜, 何希荣, 等. UPLC同时测定银黄颗粒中4种黄酮类成分含量[J]. 中国实验方剂学杂志, 2013,19(15):102-105.

QIAO Dong-ge, JI Li-na, HE Xi-rong, et al. Simultaneous Determination of Four Flavones in Yinhuang Granule by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 102-105.
乔东鸽, 吉丽娜, 何希荣, 等. UPLC同时测定银黄颗粒中4种黄酮类成分含量[J]. 中国实验方剂学杂志, 2013,19(15):102-105. DOI： 10.11653/syfj2013150102.

QIAO Dong-ge, JI Li-na, HE Xi-rong, et al. Simultaneous Determination of Four Flavones in Yinhuang Granule by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 102-105. DOI： 10.11653/syfj2013150102.

摘要

目的: 建立银黄颗粒中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的UPLC含量测定方法。方法: 采用ACQUITY UPLC H-Class超高效液相色谱仪

ACQUITY UPLC BEH C18色谱柱(2.1 mm× 50 mm

1.7 μm);流动相甲醇-0.2%磷酸水溶液

梯度洗脱

流速1 mL·min-1

检测波长274 nm

柱温30℃。结果: 黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的线性范围分别为1.09~21.8

0.340~6.80

0.116~2.32

0.102~2.04 μg

加样回收率分别为100.8%(RSD 0.32%)

97.1%(RSD 0.95%)

101.5%(RSD 1.6%)

98.7%(RSD 0.059%)。结论: 该方法简便、快速

分离效果好

可为全面控制银黄颗粒的质量提供参考。

Abstract

Objective: To develop an UPLC method for determining the content of four flavones in Yinhuang granule. Method: The analysis was performed on an ACQUITY UPLC BEH C18column (2.1 mm×50 mm

1.7 μm) eluted with methanol and water containing 0.2% phosphoric acid as mobile phases in a linear gradient mode. The flow rate was set at 1.0 mL·min-1

the column temperature was set at

and the detection wavelength was set at 274 nm. Result: The linear ranges were 1.09-21.8 μg for baicalin

0.340-6.80 μg for wogonoside

0.116-2.32 μg for baicalein

0.102-2.04 μg for wogonin respectively. The average recoveries of the four flavones were 100.8% (RSD 0.32%)

97.1% (RSD 0.95%)

101.5% (RSD 1.6%)

98.7% (RSD 0.059%) respectively. Conclusion: The method appeared to be simple

accurate and reliable

which can be used for quality control of the four flavones in Yinhuang granule.

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