Objective: Isolation and identification of saponins from Gocaekmbaw before and after heat-processing was studied. Method: The leaves of Gocaekmbaw were steam-heated at 125℃ and 0.24 MPa for 3 h

then extracted with 80% ethanol for 3 h. The extracts were isolated with chromatography using HP-20

silica gel and reversed ODS column. The constituents isolated from Gocaekmbaw before and after heat-processing were identified with 1H-NMR

13C-NMR and LCMS-IT-TOF spectra. Result: Two dammarane-type saponins were isolated from the original Gocaekmbaw. And four different compounds were got from the heat processed Gocaekmbaw. They were identified as gypenoside XLVI

gypenoside LVI

gypenoside L

gypenoside LI

damulin B and damulin A

respectively. Conclusion: Sugar chains in C20 position of gypenoside XLVI and gypenoside LVI were hydrolyzed by heat-processing to change the constituents of Gocaekmbaw.