Objective: To develop a GC-FID method for determination of borneol concentration in mice tissues and investigate its tissues distribution after oral administration. Method: Wholebrain

heart

liver

spleen

lungs and kidneys were quickly isolated by executing mice at 1

3

5

10

20

30

60

90

120 min after oral administration of borneol with a dosage of 30.0 mg·kg-1.These tissues were extracted with ethyl acetate and detected by GC-FID

octadecane was used as an internal standard. Result: Calibration curve was good linear

extraction recoveries

RSD of within-day and between-day with low

medium and high concentration were satisfied for analysis requirements of biological samples.Borneol distributed in all major organs after administration with the order of liver>kidney>heart>brain>spleen>lung

Tmax in liver

lung and spleen was earlier than in heart

brain and kidney. Conclusion: Established GC-FID could be applied to determination of borneol in tissues.There were differences of borneol distribution in each tissues

it mainly distributed in abundant blood-supply tissues such as liver

kidneys

heart and brain.