ZHANG Han, WANG Wei-ming, LI Yan-wu, et al. Effect of Kuijieling Decoction Contained Serum on Cell Proliferation in Caco-2 Cell Injury Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 167-170.
ZHANG Han, WANG Wei-ming, LI Yan-wu, et al. Effect of Kuijieling Decoction Contained Serum on Cell Proliferation in Caco-2 Cell Injury Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 167-170. DOI: 10.11653/syfj2013150167.
Objective: To investigate the effect of Kuijieling decoction contained serum on cell proliferation in Caco-2 cell injury model. Method: Rectal instillation of trinitrobenzenesulfonic acid (TNBS) was used to establish the rats model of ulcerative colitis
3 days later blood simples were collected and serum was separated
which was used to intervene Caco-2 cells for a period of time to simulate cell inflammatory injury model
the effect of different concentrations of Kuijieling decoction contained serum oncell proliferation was observed by the method of MTT. Result: Compared with the model serum group
10%
5%
2.5%
1.25%
0.625% of Kuijieling decoction contained serum promoted proliferation
especially 10% of Kuijieling decoction contained serum was best(P<0.05). Conclusion: Kuijieling decoction contained serum could promote the proliferation of Caco-2 cells after injury.