Objective: To observe effects of puerarin and oleanolic acid on blood glucose

blood lipid

superoxide dismutase (SOD)

malondialdehyde(MDA)

advanced glycation end products (AGEs)

serum hypersensitive C-reactive protein (hs-CRP) and pancreatic histopathology in rats with type 2 diabetes mellitus(T2DM)

and to approach their pharmacological mechanism. Method: T2DM rats induced by high calories diet and intraperitoneal injection of streptozotacin(STZ) were randomly divided into five groups:diabetes mellitus (M)group

melbine(D) group

puerarin(P) group

oleanolic acid group(OA) and puerarin with oleanolic acid(POA)group. Fasting blood glucose (FBG) and 2-hour postprandial blood glucose (P2hPG) of rats in each group were measured before treatment and after 2

4

6 weeks of administration. After 6 weeks

blood simples were collected for measurement on blood glucose

as well as following indexes

cholesterol (CHOL)

triglyceride (TG)

HDL-cholesterol (HDL-C)

LDL-cholesterol (LDL-C)

hs-CRP

SOD

MDA and AGEs. Pathological changes were observed under light microscope by HE staining. Result: All indexes were inhibited to certain degrees in every treatment group except HDL-C and SOD. The level of LDL-C in POA group decreased more significantly than that in the P group and OA group(P<0.05)

meanwhile

the injury pancreas tissue was improved significantly. Conclusion: The puerarin and oleanolic acid could markedly improve glucose and lipid metabolism in experimental type 2 diabetic rats. It may prove that puerarin with oleanolic acid can relieve the degree of oxidative stress reaction through antioxidant activity and suppressing inflammatory reaction. In terms of decreased LDL-C and improvement of the structure of islet cells

the effects in POA group are more stronger than those in P and OA group.