Objective: To study the anti-aging effect and mechanism of Shouzhangshen-37 pill from Mongolian medicine on subacute aging mice. Method: One hundred and twenty ICR females were categorized into six groups randomly:normal control group

aging model group

vitmin E groups (VE) high

middle

low doses of Shouzhangshen-37 pill groups. Except the normal control group

all other groups were injected D-galactose (120 mg·kg-1) once per day

lasting 7 weeks

to established subacute aging model. The high

middle

low doses of Shouzhangshen-37 pill groups were ig administered Shouzhangshen-37 pill respectively for 0.5 mL each mouse (2.4

1.2

0.6 g·kg-1 prepared with normal saline)

VE group was ig administered VE same volume (38.9 mg·kg-1

prepared with normal saline) for 7weeks. After the treatment

the anti-aging effect and mechanism were evaluated by observing the learning and memory a bility by step-through test

calculating indexes of brain

thymus

spleen

taking brain tissue

measuring the content of SOD

CAT and MDA and observing the changes of pathomorphism of cerebral tissue in mice by HE coloration. Result: Compared with model group

the Shouzhangshen-37 pill improved memory ability in mice [incubation period (152.22±38.16)

(185.75±35.03) s

(P<0.01)]

enhanced the indexes of brain[(10.43±1.66)

(13.39±1.39) mg·g-1

(P<0.01)]

thymus[(0.91±0.18)

(1.21±0.20) mg·g-1

(P<0.01)]

spleen [(3.63±0.55)

(4.76±0.82) mg·g-1

(P<0.01)]

improved the ability of SOD [(156.9±14.5)

(180.6±14.7) U·mg-1

(P<0.01)] and CAT [(5.24±0.51)

(7.14±0.72) U·mg-1

(P<0.01)] in brain tissue

decreased content of MDA in brain tissue [(4.31±0.47)

(3.17±0.73) nmol·mg-1

(P<0.01)]. Conclusion: Shouzhangshen-37 pill has an anti-aging effect in subacute aging mice. The mechanism may enhance antioxidant activity in brain tissue

decrease content of MDA in brain tissue

protect the brain nerve cells

improve memory ability in mice.