附子理中汤对腹泻型肠易激大鼠模型结肠组织中TLR4 mRNA表达水平的影响

武志娟; 张志敏; 张大鹏; 谢文娟

doi:10.11653/syfj2013150218

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附子理中汤对腹泻型肠易激大鼠模型结肠组织中TLR4 mRNA表达水平的影响

更新时间：2024-01-04

- 附子理中汤对腹泻型肠易激大鼠模型结肠组织中TLR4 mRNA表达水平的影响
- Expression of TLR4 mRNA in Colon Tissue of Rat Diarrhea Type Irritable Bowel Model by Fuzi Lizhong Decoction
- 中国实验方剂学杂志 2013年19卷第15期页码：218-221
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省中医药局资助项目(20111261)
- DOI：10.11653/syfj2013150218
  中图分类号：
- 纸质出版日期：2013
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武志娟, 张志敏, 张大鹏, 等. 附子理中汤对腹泻型肠易激大鼠模型结肠组织中TLR4 mRNA表达水平的影响[J]. 中国实验方剂学杂志, 2013,19(15):218-221.

WU Zhi-juan, ZHANG Zhi-min, ZHANG Da-peng, et al. Expression of TLR4 mRNA in Colon Tissue of Rat Diarrhea Type Irritable Bowel Model by Fuzi Lizhong Decoction[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 218-221.
武志娟, 张志敏, 张大鹏, 等. 附子理中汤对腹泻型肠易激大鼠模型结肠组织中TLR4 mRNA表达水平的影响[J]. 中国实验方剂学杂志, 2013,19(15):218-221. DOI： 10.11653/syfj2013150218.

WU Zhi-juan, ZHANG Zhi-min, ZHANG Da-peng, et al. Expression of TLR4 mRNA in Colon Tissue of Rat Diarrhea Type Irritable Bowel Model by Fuzi Lizhong Decoction[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 218-221. DOI： 10.11653/syfj2013150218.

摘要

目的: 观察附子理中汤对腹泻型肠易激(D-IBS)大鼠模型结肠组织中toll样受体4(TLR4)表达水平的影响。方法: 健康SPF级大鼠50只随机分为正常组、模型组、匹维溴铵组、附子理中汤高、低剂量组

用束缚应激加大黄ig方法联合复制D-IBS大鼠模型

造模成功后附子理中汤高、低剂量组(30

15 g·kg-1)、匹维溴铵(30 mg·kg-1)ig给药

1 次/d

连续14 d。采用荧光定量PCR方法观察该模型大鼠结肠黏膜TLR4 mRNA的表达情况。结果: 模型组大鼠肠黏膜的TLR4的表达(1.229±0.172)高于正常对照组(1.109±0.062)(P<0.01)。而附子理中汤的干预可使大鼠肠黏膜的TLR4的表达趋于正常

与正常组无明显差异。附子理中汤高(1.068±0.056)

低(1.067±0.071)剂量组之间没有显著差异

匹维溴铵组(1.205±0.290)与正常组相比TLR4的表达增多(P<0.01)。结论: 附子理中汤可能通过增强整体机体耐受性

降低TLR4的表达

减少免疫应答

来维持肠道的稳态。

Abstract

Objective: To observe the toll like receptor(TLR)4 mRNA expression decoction on rat diarrhea type irritable bowel syndrome (D-IBS)colon tissue model by Fuzi Lizhong(FZLZ) decoction. Method: SPF 50 rats were randomly divided into normal group

model group

FZLZ high and low dose group(30

15 g·kg-1)

pinaverium bromide group(30 mg·kg-1). IBS rat model was made by restraint stress combined with ig rhubarb. After modeling

corresponding drugs were given at the dose

once a day for 14 days. TLR4 mRNA expression was observed through PCR method. Result: The expression of TLR4 in the model group (1.229±0.172)was higher than that in the control group(1.109±0.062)(P<0.01). While the expression of TLR4 in the FZLZ decoction was similar to normal groups

there was no significant difference between the high (1.068±0.056) and low (1.067±0.071)dose group

pinaverium bromide group(1.205±0.290) compared with the normal group TLR4 expression increased (P<0.01). Conclusion: FZLZ decoction may maintain intestinal homeostasis by enhancing the whole body tolerance

and decreaseing the expression of TLR4 and immune response.

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