Objective: Equivalence studys of refined extract and crude solution of anti-radiation pneumonia decoction (ARPD) on radiation-induced lung injury. Method: Refined extract of ARPD was made by alcohol purification from crude solution. Experiment group: model group

crude solution treatment group

refined extract treatment group and control group. Rats in model group

crude solution treatment group and refined extract treatment group were thoracically exposed to 6 MVX-rays at a dose of 17 Gy. The rats were treated with different solutions by gastrogavage once a day just after X-ray exposure. Rats in crude solution treatment group and refined extract treatment group were treated with 3.5 g·mL-1 crude and refined ARPD respectively

and rats in model group and control group were gavaged with 0.9% sodium chloride

each time 10 mL·kg-1. The rats were sacrificed at the day of 7

15

30

45 and 60 after X-ray exposure. The levels of superoxide dismutase(SOD)

malondialdehyde (MDA) and hydroxyproline (HYP) in lung tissues and sera were measured simultaneously. Result: ①Pathological examination of lung tissue of model group revealed inflammatory changes and proliferation of collagenous fibers

in lung tissue and serum SOD activity decreased

while the content of HYP and MDA increased

much higher than the concurrent control group (P<0.01)

with a statistically significant difference; ②The inflammatory reactions and collagenous proliferation in crude solution treatment group and refined extract treatment group were much slighter

compared with the same period of model group

but there was no difference between these two treatment groups;③The reduction of SOD activity and the rises of content of MDA and HYP in the model group severely greater than the crude solution treatment group and refined extract treatment group(P<0.01)

while no significant difference between two treatment groups. Conclusion: ①ARPD may reduce the inflammatory reaction of radiation-induced lung injury and reduce pulmonary fibrosis caused by radiation injury. ②There are similar anti-radiation effects on treating lung injury between refined extract and crude solution of ARPD.③The method of water extraction and alcohol precipitation is viable in purifying ARPD.