Objective: To explore the mechanism of the modified Yinchenhao decoction(YCHT) in treating acute liver injury. Method: Seventy-two female SD rats were randomized into six groups with 12 in each group:blank control group

model group

compound glycyrrhizin tablets group

and low

middle and high dosage group of supplemented Yinchenhao decoction. All the rats were fed with common feed and clean water in the 15-20℃ and humidity level at 60%-80% of laboratory for seven days

seven days later

single intraperitoneal injection of D-GalN at a dosage of 600 mg·kg-1 was administered to the rats except those in the blank control group to induce acute liver injury. Medications were given orally to each treatment group at the same time

the daily dose of compound Glycyrrhizin tablets was 15.75 mg·kg-1

and dosage of the three herbal groups was 14.7

29.4

58.8 g·kg-1 respectively. The blank control group was fed with the 0.9% physiological saline.All the medications were given at a volume of 10 mL·kg-1

twice a day for two days. 48 h after modeling

all rats were sacrificed and livers werecollected

RT-PCR and western blot were used to detect the expression of TLR-4 mRNA and TLR-4 protein in liver cells. Result: Compared with the model group

YCHT could significantly reduce the TLR-4 mRNA (P<0.01) and TLR-4 protein expression (P<0.01). Conclusion: Through reducing the TLR-4 mRNA and TLR-4 protein expression of rats liver cells

modified YCHT could effectively intervene the liver cells fibrosis.