Objective: To study the effects of Zhuling Tang on serum arginine vasopressin and γ-ENaC in adriamycin-induced nephropathy rats. Method: One hundred and ten male SD rats were randomly divided into control group

sham operation group

model group

14 days after tail vein injection of adriamycin

urine protein quantitation≥100 mg·(24 h)-1 prompt model copied successfully. The survival model group rats were again randomly divided into the model group

Zhuling Tang group

furosemide group. Respectively gavage two weeks

four weeks

six weeks

eight weeks

some rats were sacrificed and specimens of urine and kidney specimens

semi-automatic biochemical analyzer detect serum albumin

lipids

liver and kidney function

urine protein

ELISA method was use d to detect the expression of the serum acrginine(AVP)

γ-ENaC protein expression was detected by immunohistochemical. Result: ① Compared with blank group

in model group rats urine protein

creatinine

blood urea nitrogen

serum lipids

serum Na+

AVP gradually increased(P<0.05)

serum albumin gradually decreased (P<0.05);compared with the model group

Zhuling Tang could increase rat urine output (P<0.05)

lowered AVP (P<0.05);② In terms of cell morphology:in the model group

group of Zhuling Tang and furosemide groups focal segmental glomerulosclerosis

tubular protein casts

renal interstitial edema

fibroblasts and inflammatory cell infiltration were found;③ For kidney γ-ENaC:in the model rats kidney γ-ENaC show a trend of increase and then decrease

within the group

the difference was statistically significant (P<0.05)

in Zhuling Tang

kidney γ-ENaC expression was lower than the model group (P<0.05). Conclusion: The diuretic effect of Zhuling Tang may be related to downregulating the serum AVP and kidney γ-ENaC protein.