Objective: To prepare gentiopicroside nano-lipid carriers(GEN-NLC) and investigate its stability

in vitro release and properties. Method: Solvent dispersion method was used to prepare GEN-NLC.With particle size

Zeta potential and encapsulation efficiency as indexes

effects of proportion of solid-liquid lipid

drug-lipid ratio and the type and concentration of surfactant on preparation technology were investigated

morphology of prepared GEN-NLC was observed by transmission electron microscopy

its characteristics and stability was investigated. Result: Optimal prescription was as following:0.1% poloxamer 188 as surfactant

drug-lipid ratio 1: 10

liquid lipid ratio 10%.Encapsulation efficiency of prepared GEN-NLC was (38.19±1.61)%

drug loading was(3.47±0.08)%

particle size was(129.9±3.07) nm

PDI was (0.264±0.01)

Zeta potential was (-22.5±0.42) mV. GEN-NLC was spherical particles and showed monodisperse distribution

rounded appearance and uniform size.After stored at 4℃ for 30 d

indicators were not changed significantly

release of gentiopicroside 39.65% in 4 h

cumulative release amounted to 79.86% at 36 h. Conclusion: It showed that there were good physicochemical properties and stability of GEN-NLC prepared by solvent dispersion method

and had a certain degree of sustained-release long-lasting effect.