Objective: To analyze cleansing mechanism of Aconitum kusnezoffii. Method: HPLC was adopted to determine the content of mesaconine

aconitine

hypaconitine

benzoylmesaconine

benzoylaconitine

benzoylhypaconitine in A. kusnezoffii with stems

A. kusnezoffii without stems and stems of A. kusnezoffii. Result: The total content of three kind of diester alkaloids in A. kusnezoffii with stems

A. kusnezoffii without stems and stems of A. kusnezoffii was 0.59%-0.66%

0.61%-0.69%

0.39%-0.42%

respectively;The total content of three kind of monoester alkaloids was 0.09%-0.10%

0.09%-0.12%

0.06%-0.08%

respectively.Except six alkaloids chromatographic peaks

there was another common peak(peak 4)

area of peak 4 in stems of A. kusnezoffii was 55%-80% to that in A. kusnezoffii without stems. Conclusion: Content of six alkaloids was found in A. kusnezoffii without stems and stems of A. kusnezoffii was different.Stems of A. kusnezoffii was the non-medicinal parts whose content of six alkaloids was low

it could be removed.