ZHOU Xi, CHEN Pei-dong, Zhang Li, et al. HPLC Fingerprint of Shixiao San[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 73-76.
ZHOU Xi, CHEN Pei-dong, Zhang Li, et al. HPLC Fingerprint of Shixiao San[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 73-76. DOI: 10.11653/syfj2013160073.
Objective: To establish the fingerprint of Shixiao San
enhance the quality control of the method. Method: The RP-HPLC was performed on an C18 chromatography column
with acetonitrile-0.1% of TFA as mobile phase
the flow rate was 1 mL·min-1; the wavelength was set at 360 nm. The temperature of column was maintained at 30℃. Result: The common modes for the HPLC fingerprints were set up by ensuring 15 common peaks in the chromatograms. The similarity degrees in the ten batches were in the range of 0.962 to 0.996. Conclusion: The method is sensitive
accurate and simple
thereby it can be used specifically for the quality control of Shixiao San.